Aubrey Funke, the Assistant Director of the Imaging and Histology Core Facility (IHCF), welcomed the iCREATE students to the IHCF to learn how to use an extremely powerful (and extremely expensive) microscope! The Scanning Electron Microscope (SEM) can magnify 1,000,000 times - which is 1,000 better than a light microscope, which is already 1,000 time better than our eyes!
The Imaging and Histology Core Facility provides imaging services to faculty, undergraduate and graduate students, along with industry partners. They provide services in light microscopy, electron microscopy, and histology (microscopy of tissues).
Graduate Student Alyssa Talbert creates scaffolds for wound healing with elastin, collagen and water in her graduate work. But here, she is illustrating how to collect and stain cheek samples for the students to view under the light microscope.
Alyssa and Aubrey then take the students to compare the digital light microscope images with the detail they can see with the SEM. Because electrons have a very small wavelength, much smaller than the wavelength of light, the resolution using the SEM is much greater than that of light microscopes.
Aubrey and Alyssa shared a variety of SEM images with the students, from insects (see past Ugly Bug Contest winners below), to fungi, Alyssa's scaffolds, and much more!
The students spent approximately 8 hours at the IHCF learning how to use the SEM. They took copious notes they then followed explicitly, with Aubrey observing them, and they were able to run the SEM themselves on their last day of the training!
Thank you to both Alyssa and Aubrey for all the time and patient effort they put into instructing the students on the SEM. We weren't surprised to learn that Aubrey won the "Outstanding Staff Member" for NAU's Biology Department at their award ceremony on Wednesday, May 2nd. Congratulations to Aubrey!
Guest Blog Post by Tad Theimer, NAU Professor of Biology
Flagstaff is a city of science. We are literally surrounded by science. Up on Mars Hill there lies Lowell Observatory, to the north the Museum of Northern Arizona, the offices of the USGS, to the east and west the laboratories of GORE, to the south Northern Arizona University, the Rocky Mountain Research Station, TGen, the Naval Observatory, to mention but a few.
So I stand here as a scientist in a city of scientists. How many of you out there are scientists? How many the family or friends of a scientist?
It’s been said that you know you are a scientist when you wake up on a Saturday morning and think, “I could walk the dog, I could read the paper, I could go for a run, but what I really want to do is analyze that new data set, or sneak off to the lab for one more quick experiment.” And all your friends and families of scientists have seen that, you’ve seen them sneak out the door late at night or early in the morning. So we here all know that inside the breast of every scientist beats a heart as passionate, as driven, as that of any artist, musician or poet. Scientists do what they do because they can’t help themselves. They are driven by an unquenchable thirst for knowledge, by an insatiable hunger to understand the world. That relentlessness, that dogged curiosity, is something folks who haven’t been around scientists may not realize, and as a result they may underestimate the power that stands here today. Because that same passion, that same resolution, that scientists bring to doing science, scientists will also bring to defending science. And that is why we stood here last year, and why we stand here today and why we will be here next year, and the year after that, and the year after that!
So we here in Flagstaff understand what science is, why it is important, and that we must help others understand the important role science plays in our lives. The speakers who went before me articulated that very well. But there is another point about science that we have to make folks understand. Scientists follow data to whatever truth they may lead, regardless of the implications that truth may have. And so scientists sometimes discover inconvenient truths, truths that make us have to question the way behave toward this earth, toward each other. Truths that are inconvenient because they come with costs. The cost of making sacrifices today so that our children and grandchildren can have a decent world to live in tomorrow. This is an important role scientists play, and
I have been trying to think of a simple analogy to help folks understand that important role of science, so let me try this out on you:
Let’s imagine that my inconvenient truth is that I only have $10 in my bank account. But because of this magical piece of plastic called a credit card, I can buy a car, I can buy fancy food and delicious drinks. And some of my friends will support me in ignoring my inconvenient truth because they like to ride in my car and drink my drinks and eat my food. But one or two of my friends will take me aside and say “Tad, what are you doing? You’re acting crazy! You only have ten bucks! If you keep this up you’re heading for financial ruin!” Now we all know which one of those friends is the most valuable. It’s the one with the courage to stand up and tell me the truth even though I didn’t want to hear it. And that’s what scientists do! They are the friends who stand up and tell us the truth even when we don’t want to hear it!!
We are passing through dark days for science. Honesty, reliability, consistency, responsible conduct. These are the cornerstones of science. These are also the foundations of a civil society. Yet every day these ideas are mocked, denigrated, cast aside! We live in a time when integrity has been replaced with irresponsibility, where falsehoods hold the same credence as facts. It is no wonder that we sometimes feel dazed, in a world turned upside down.
These are dark days, but we have seen darker. When I am most in despair for this world, I am reminded of Galileo, that great scientist who dared to follow his data to an inconvenient truth, that radical idea that the earth was not the center of the universe, fixed and immovable, but instead moved around the sun in its orbit. Today that seems like a ridiculously harmless fact, that the earth goes around the sun, but at the time, it was a very inconvenient truth, for it flew in the face of religious dogma. So at the age of 70, Galileo was dragged from his home, thrown in prison and eventually brought before the Inquisition in Rome, forced to kneel and to recant his life’s work, to state that the Earth was immobile and did not move in its orbit. But the story is told that as Galileo walked out of that room, he whispered under his breath, “and yet, it moves!” And so might we say to those who deny climate change today, “and yet, it changes!”
You can ignore the truth for a while. You can walk away from the Paris climate accords, you can tell your administrators to strike out all references to human-caused climate change. You can confuse the electorate by saying climate change is still debated, that there is no consensus. You can ignore the National Academy of Sciences and 17 other scientific societies that have stated that human-caused climate change is real and needs to be addressed. You can ignore all that and build yourself a beautiful house of cards. But eventually that house of cards will fall. Scientists know this. Scientists understand the meaning of that old Buddhist saying: “There are three things in this world that cannot long be hidden: the sun, the moon, and the truth”!
Make no mistake, the walls of ignorance are strong, especially when reinforced by girders of greed and self-interest. But history has shown us that that stone of ignorance will yield to the cold, hard steel of science-based fact. It is for us today to follow in the footsteps of all those scientists and believers in science that went before us, to pick up those hammers of steel and bring them down on that rock of ignorance, knowing all the while that those walls will not fall to one blow, or to a thousand, but the point is to keep on hammering, to keep those hammers ringing. So I say, make those hammers ring here in Flagstaff, but also make ring so that they can be heard down in the statehouse in Phoenix. Make those hammers ring here in Flagstaff, but also make them ring so that they echo in the halls of congress back in Washington. Make those hammers ring here in Flagstaff, but most importantly, make those hammers ring so that they rattle the very walls of the White House! Make those hammers ring!
By Dave Engelthaler, Associate Professor at the Translational Genomics Research Institute and the Chair of the Northern Arizona Leadership Alliance. This column was adapted from the keynote speech, given by the author, at Science Foundation Arizona's "Giving a Voice to STEM" Conference at NAU on September 30, 2016.
I have often referred to Flagstaff as the Shining City on Arizona’s Hill. It is no accident that I borrow this phrase from the famous, precisely American, ideal of a “Shining City on a Hill”. The early pilgrims imagined that they could create such a community for themselves after escaping the historical norms of European controls on destiny.
Three hundred years later John F. Kennedy reminded of this founding ideal, stating that the world was watching our shining city and that we must live up to our promise; shortly there after, we embarked on one of the greatest journeys of all time and put a man’s foot on the moon (Flagstaff had something to do with that, more on that below).
Twenty years later, Ronald Reagan again reminded us of this American City on a Hill ideal; and while we may not often remember Reagan as a champion of science, he was convinced during his tenure to not only not cut the budget of the National Science Foundation, but rather double it, before he left office. But, as under Kennedy and Reagan and other presidents in before and after, no matter what our economic and cultural condition, we have always led the way in advancing humanity through the sciences.
It is this ideal that convinces me that in Flagstaff, we are a Shining STEM City on Arizona’s Hill.
In August of 2012, a group of Flagstaff Leaders, Businessmen, Educators, Scientists, and Concerned Citizens gathered in the woods on the base of the San Francisco Peaks. This group coalesced around the idea that Flagstaff is a STEM-rich City and that we as a community, businesses and schools, elected leaders and CEOs, teachers and families, needed to collectively band together to bring this rich surrounding to bear on the education of our children and enrich our communities.
There, up on our Hillside, we all mutually pledged our time, talent and resources towards making the STEM City ideals happen. In short – our goal was to have the most STEM literate graduates living and working in a thriving STEM-based economy.
We also had a unofficial motto for the day: “Dare Mighty Things”, which we borrowed from the Jet Propulsion Laboratory, who had, just the preceding night, coordinated the landing of the Curiosity Rover on Mars (and again, Flagstaff had something to do with this mission). And we both stole that from Teddy Roosevelt’s famous “Far better it is to dare mighty things” speech.
Historian, Fredrick Jackson Turner, just a few years before TR’s famous speech, gave us his “Frontier Thesis”, and proclaiming that with the end of the American Frontier, so might be the end the American spirit. While Jackson aptly, and controversially, linked Americanism and American spirit to the discovery and exploration of the American Frontier, I feel that he missed the mark in not understanding the new frontiers that we would identify and explore.
Our increased understanding and use of science and engineering opened up brand new frontiers, beyond land and sea.
One such Frontier, The Space Frontier, was no longer a pastoral landscape to watch from afar. Our STEM City has been at the forefront of the exploration of this new frontier, from the discovery of Pluto, to the training of Apollo astronauts in our backyard, to the camera control of the Mars Rover from our USGS facility, and now finally to the deep space explorations through our Discovery Channel telescope, providing insight into the beginnings of our universe and images of a frontier previously unseen.
Likewise, Flagstaff is home to TGen and the new Pathogen and Microbiome Institute at NAU, where some of the brightest minds are exploring another previously unseen universe – the microbiome. Every day, scientists in Flagstaff are embarking on the incredible journey into the human microbiome – the unseen ecosystem of bacteria and viruses and fungi that live on and in the human body. We are trying to understand how these microbes live, compete, collaborate and otherwise interact during our healthy and disease states. We, ourselves, our bodies, are the new frontier – and again that frontier exploration is here in our STEM City.
And we could go on about the new frontiers ventured by W.L.Gore engineers and SenesTech scientists and MNA paleontologists and Park Service geologists. The frontier is here in our STEM City and some of the greatest pioneers are the trainers of our next generation– the teachers and education professionals of our great public, charter and private schools. Most are ready, willing and able to interact with all of these resources; and some, like former STEM City Teacher of the Year Jillian Worssam, just kick down the door and say: “Let’s do this thing!”
Our STEM City is Worssam’s wildly successful Scientists in the Classroom. It is the Flagstaff Festival of Science (the longest running one in the country). We are the Coconuts; we are the Annual STEMMY'S Awards Ceremony; the STEM Art Competition; and the Super Bowl of STEM in the Dome event (where upwards of 8% of Flagstaff turns out!); we are the Space Station Science Experiment and the High Altitude Balloon Launches; and the superstar Killip Kindergarten Chess team that likes to challenge our Mayor. We are seventh-grade girls wearing lab coats inside a world-class research lab and we are a group of high schoolers rafting down our majestic Canyon to learn our geologic past. We are the Chamber Coding Camps. We are grad students teaching and learning in the K-12 classroom. We are parents, students and teachers on a hill having a star party. We are, in a phrase, America’s First STEM Community.
Arizona, and the rest of the country, is watching our shining STEM City
and we must live up to our promise.
Guest post by Lisa Winters, formerly of Arizona Game and Fish, and presently a STEM VISTA Member with the Grand Canyon Trust
The best ten days of the year, the Flagstaff Festival of Science, is in full swing. And this year, we had the first BioBlitz at Francis Short Pond! Organized by Rocky Mountain Research Station, Willow Bend Environmental Education Center, and Friends of the Rio de Flag, the BioBlitz was an opportunity for students, teachers, and the general public to work in collaboration with biologists, naturalists, and other scientists to complete a biological inventory of the plants, animals, and organisms that live in or near the pond.
Thanks to Lisa Winters, left, of Grand Canyon Trust, and Zack Zdinak, right, of Life Drawing and Education
Stations were set up around the pond that collected information about water quality, aquatic insects, birds, plants, and fish. Over 260 students from Marshall Elementary, Flagstaff Junior Academy, and Mount Elden Middle School measured the temperature and dissolved oxygen of the water, used microscopes to identify the aquatic invertebrates they caught, wandered the pond in search of common plants, used binoculars to spot ducks and red-winged blackbirds, fished for rainbow trout, and then pulled together what they learned by constructing a life cycle diagram of an organism of their choice. In the afternoon, many community members got the same chance to explore this unique ecosystem in their backyard while contributing to the survey data collection.
Photos show Alice patiently fishing, the excitement of the catch, and measuring for data prior to release!
Additional partners of the event include the City of Flagstaff Sustainability Section, The Museum of Northern Arizona, Grand Canyon Trust, local illustrator Zack Zdinak, and more! The event was made possible through a generous grant from the National Geographic Education Foundation and the AZ Game and Fish Heritage Grant. Thank you all for the great contributions to citizen science and education in Flagstaff!
Flagstaff STEM Coordinator