Aubrey Funke, the Assistant Director of the Imaging and Histology Core Facility (IHCF), welcomed the iCREATE students to the IHCF to learn how to use an extremely powerful (and extremely expensive) microscope! The Scanning Electron Microscope (SEM) can magnify 1,000,000 times - which is 1,000 better than a light microscope, which is already 1,000 time better than our eyes!
The Imaging and Histology Core Facility provides imaging services to faculty, undergraduate and graduate students, along with industry partners. They provide services in light microscopy, electron microscopy, and histology (microscopy of tissues).
Graduate Student Alyssa Talbert creates scaffolds for wound healing with elastin, collagen and water in her graduate work. But here, she is illustrating how to collect and stain cheek samples for the students to view under the light microscope.
Alyssa and Aubrey then take the students to compare the digital light microscope images with the detail they can see with the SEM. Because electrons have a very small wavelength, much smaller than the wavelength of light, the resolution using the SEM is much greater than that of light microscopes.
Aubrey and Alyssa shared a variety of SEM images with the students, from insects (see past Ugly Bug Contest winners below), to fungi, Alyssa's scaffolds, and much more!
The students spent approximately 8 hours at the IHCF learning how to use the SEM. They took copious notes they then followed explicitly, with Aubrey observing them, and they were able to run the SEM themselves on their last day of the training!
Thank you to both Alyssa and Aubrey for all the time and patient effort they put into instructing the students on the SEM. We weren't surprised to learn that Aubrey won the "Outstanding Staff Member" for NAU's Biology Department at their award ceremony on Wednesday, May 2nd. Congratulations to Aubrey!
Colleen Hopkins, Telehealth Coordinator for North Country Health Care (NCHC) shared an interactive lesson on NCHC’s Telehealth program with the iCREATE class on March 15, 2018.
NCHC reaches across all of Northern Arizona, stretching 500 miles from Nevada to New Mexico, and has 23 Access Points for care across this vast region. The mission of NCHC is to provide accessible, affordable, comprehensive, quality primary healthcare in an atmosphere of respect, dignity, and cultural sensitivity. The health and well-being of patients and community alike are promoted through direct services, training/education, outreach, and advocacy.
The NCHC Telehealth program uses video conferencing technology to link providers and their patients, as well as educators and health care consumers, to a comprehensive continuum of care. Using this technology, they can reduce the isolation of providers and their patients within rural communities. They can also save a lot of time. For example, a patient can get immediate information and help for behavioral health, diabetes, nutrition advice and more, without a practitioner needing to make a 6-hour drive.
The students watched a video about Project ECHO (Extensions for Community Healthcare Outcomes) by Dr. Sanjeev Arora from the University of New Mexico. He shared this collaborative model of medical education and care management that empowers clinicians everywhere to provide better care to more people, right where they live. The students then practiced using the system to watch their own heart rate on the monitor (photo above) that a remote doctor or nurse would be able to observe and hear real-time. They also used another monitor to see their ear drum (photo below).
Colleen ended the field trip with a tour of the NCHC facilities. Students got to see many areas in the clinic and see the dedication of the entire community working at North Country Health Care. Thank you, Colleen!
As stated on their website: The Ancient DNA Lab provides support for researchers working with ancient, historical, forensic, or other sensitive (low DNA quantity/quality) genetic samples. Established by the School of Forestry’s Carol Chambers and Faith Walker in 2016, the Ancient DNA Lab is physically isolated from other genetics labs on campus and adheres to rigorous quality control measures to prevent contamination, both of which are internationally recognized standards for the early stages of ancient DNA handling and processing.
The Ancient DNA Lab is sterilized (left) and ready to use in 2018.
The 2017 iCREATE class (right) was able to go into the lab between cleanings.
Some of the projects the lab has been involved with include a 10,000 year old bat, research using both extant (living) bats and bat guano, the 8,500 year old bison (below), and work with extant wombats in Australia.
Undergraduate Research Assistant Sam Hershauer presented some basic information on how DNA is extracted, amplified and analyzed in the aDNA Lab. Sam is working on collaborative research project on DNA from organisms in Alaskan lake sediments going back thousands of years. This collaborative project is with the Arctic Lakes Project headed by Dr's. Nick McKay and Darrell Kaufman in the School of Earth Sciences and Sustainability.
Thank you to Faith, Colin and Sam for sharing your hidden treasure with the iCREATE students!
Guest Blog Post by Nick Siskonen, AmeriCorps STEM VISTA, CAVIAT
Every student enrolled in our CAVIAT programs has the opportunity to participate in a Career and Technical Student Organization (CTSO). These organizations provide scholarships, competitions, leadership opportunities and so much more that enriches the life and learning of our students.
Some of our students are traveling to the Arizona state competition hosted by the CTSO, HOSA - Future Health Professionals. HOSA is an international organization focused on developing character and technical skill competencies for members, to uplift current and future people in the health professions.
Our students are there to compete in a variety of subject matters. Madison Stump, of the Medical Professions program, is competing in the Behavioral Health event. Cylie John, also in the Medical Professions program, is taking the Medical Law and Ethics test. Dakota Palmer, of the Veterinary Assistant program, is doing the Veterinary Science skills test. Zachary Ashland and Elizabeth Strones, from the Bioscience program, are both taking part in the Medical Innovation event, and their classmate Antonia Green is taking the Biomedical Laboratory Sciences test.
To qualify for this week's state competition, students took an online test which covered a wide variety of topics from their program's curriculum. Only top scoring students are allowed to attend the state competition and earn the chance to move on to the international competition at the International Leadership Conference, which takes place at the end of June.
The state competition this year is taking place in Tucson, from April 2nd through April 4th. Let's cheer them all on to victory!
And not to be left out of the fun, this week our Fashion Design and Merchandising program is headed off to Los Angeles! They're going to visit the Fashion Institute of Design & Merchandising and the LA fashion district. They've been fundraising for this trip all year, and all the hard work has finally paid off.
Eleven high school students in the CAVIAT iCREATE bioscience class publicly presented their unique solutions to the authentic problem of tracking and reporting influenza-like illnesses in Coconino County. The presentations were held on March 7th at NAU's Center for Science Teaching and Learning. The students are in the second semester of this college-level course that earns credits from both Coconino Community College plus from Coconino High School or Flagstaff High School. The class meets after school for 2.5 hours each day from Monday to Thursday to learn the CTE (career and technical education) bioscience standards through an epidemiologic lens and with rich community involvement. Community partners include Coconino County Public Health Services District, North Country HealthCare, Northern Arizona Area Health Education Center, Northern Arizona Healthcare and TGen North. The students also gained assistance from Corryn Smith in using GIS technology for their reports.
Instructors Dr. Aaron Tabor and Robert (Bobby) Woodruff co-teach the class at NAU. Both have extensive experience in research and education. They also include additional community partners for in-class presentations and field trips. Students have toured the Science and Health Building at NAU, the Center for Ecosystem Science and Society (ECOSS) laboratories, the Clinical and Pathology Laboratory at Flagstaff Medical Center, and more!
The students study disease-causing agents as then use the tools necessary to determine what microbes cause the illnesses. The class includes biosafety skills, microbiology techniques, DNA extraction, separation and analysis. Students take an end-of-year test to demonstrate their knowledge and skills.
Congratulations to all the students! And thank you to the community members that attended their presentations! NAU Provost and Vice President for Academic Affairs Dan Kain (Left), FUSD Assistant Superintendent for Curriculum and Instruction Mary K Walton (Center), and FUSD Superintendent Mike Penca (Right), all came to hear the student presentations and ask them questions about their unique solutions to this authentic community problem.
The 12 CAVIAT students in the iCREATE high school bioscience class at NAU are learning some basic Geographic Information Systems (GIS) skills to help track patient health for their epidemiology projects. Corryn Smith patiently teaches the students the basics of GIS. Corryn has presented to the class twice this fall, and will certainly be helping them again as they prepare their group projects.
Corryn is an Instructor for the Geography, Planning, and Recreation department at NAU. She received her MS in Applied Geospatial Sciences with a Planning and Recreation Emphasis in May 2017. Her Master's thesis research looked at using geospatial technologies to locate travel networks (Forest Service roads and trails) in Flagstaff. Her interests include: geospatial technologies and recreation, geospatial technologies and sustainable land management, GIS in education, and Python Programming for Women and Minorities.
Thank you Corryn for your friendly and professional help!
Guest Blog Post by Tessa Palazzolo, Mechanical Engineering at NAU
On November 13th, three schools competed in the second ever KidWind Challenge wind turbine design competition. Little Singer Community School, Coconino High School and Northland Preparatory Academy arrived with a total of 14 teams eager and ready to compete. The students were scored based on their wind turbine power output and their overall knowledge on wind energy, along with real life applications of the wind farm industry.
The event consisted of other ongoing challenges such as sail car designs, energy principle questions in jeopardy, and testing out the Human Powered Vehicle (HVP) designed by NAU’s American Society of Mechanical Engineers (ASME). The NAU ASME volunteers were also present at the competition to engage in questions related to college and the guidelines of becoming an engineer with the students. The overall experience of the KidWind Challenge provides a learning experience in hands-on creativity and allowing the students to be inspired with science, engineering and renewable energy.
The Little Singer 5th and 6th grade students were led by teacher Tom Tomas, and were doing an entire unit that incorporated literacy as well as engineering. Students are reading The Boy Who Harnessed the Wind: Creating Currents of Electricity and Hope, by William Kamkwamba (with Bryan Mealer) that shares the remarkable story of his youth in Malawi, Africa—a nation crippled by intense poverty - and how, with tenacity and imagination, he built a better life for himself, his family, and his village.
The students are also studying biomimicry, an approach to innovation that seeks sustainable solutions to human challenges by emulating nature's time-tested patterns and strategies. You can see the biologic patterns in their blade designs above and below.
A big STEM City Thank you to Project Director Karin Wadsack, Lead Organizer Tessa Palazzolo and all the ASME students at NAU that came out to help! Also, thank you to the Boys and Girls Club of Flagstaff for hosting the KidWind Challenge again!
Dr. Darlene Lee, an anatomical and clinical pathologist with Northern Arizona Healthcare, led a tour of Clinical and Pathology Laboratory at Flagstaff Medical Center to the high school iCREATE students. The iCREATE class is unique in the pronounced role of community members to the success of the class. Dr. Lee gave a tour last year, highlighted here, and it was one of the most memorable tours for the class. She made this one even more hands-on with additional partners from FMC.
Garn Bailey, Pathology Assistant, once again thoroughly engaged the students with a variety of human organs that he prepares for the pathologist to more critically analyze as needed. He dissected a gall bladder to show the gall stones, as well as a kidney that showed a cancerous area. He also went through the entire process of preparing thin sections from larger tissues and organs for analysis.
The surgeons in the hospital rely on the Clinical and Pathology Laboratory to rapidly prepare and assess samples while some patients are in surgery; to make sure they have removed all cancerous cells, or to determine the specific pathogen. When that occurs, tissues are quickly put on ice and are then sliced thinly in a cryostat, which keeps the tissues frozen. They are then rapidly stained and assessed by the pathologist. This entire process can occur in 20 minutes so the surgeon is able to receive the information during surgery to improve the patient's outcome.
The students then looked at blood samples with Dr. Lee. They were able to identify components of blood including white blood cells (leukocytes), red blood cells (erythrocytes), and platelets (thrombocytes). Platelets are tiny blood cells that help your body form clots to stop bleeding. She also showed them the image of the cancerous kidney tissue from the kidney Garn had shown them earlier.
Students observed the hospital's blood supply with Jordan Ippolito, a Medical Lab Scientist at FMC. She told the students that the shelf life of platelets is only 5 days, while frozen plasma is good for a year. Red blood cells are only functionally sound for 40 days because their ability to carry oxygen (their primary role) is impaired after that. The importance of donating blood cannot be overstated!
Two students volunteered to have their blood drawn so they could determine what blood type they are. Phlebotomist Troy Schafer cracked jokes with the students to put them at ease.
Jordan then had the remaining students look at blood sample directly through the microscope so they could put their earlier practice to use.
The final activity was to discuss what having blood types A,B, AB, or O really means, and then to complete the analysis of the blood types using the antigens that can cause blood to clot, depending on what the blood type is.
Thank you again for the fascinating and educational tour of the Clinical and Pathology Laboratory at FMC! Special thanks to Dr. Darlene Lee, Garn Bailey, Jordan Ippolito and Troy Schafer! You are living up to your mission and goal; you definitely wowed us!
Melissa (Lissy) Enright, a graduate student in ECOSS at NAU, shared her research on the hydraulics (water movement) of giant redwood trees with the high school students in the iCREATE class at NAU.
Lissy shared some of her background with the students including working on an oceanographic vessel with the Sea Education Association, working for the National Park Service in Hawaii, and for American Conservation Experience as an AmeriCorps Member in both Flagstaff and Alaska! She also worked with Honko, an NGO (non-governmental organization) working in partnership with the coastal communities of Madagascar on mangrove conservation. She showed the students the website for the Student Conservation Association where you can search through their list of opportunities, including those for students under 18 years old!
Lissy's experience helped her to get a "real job" working with the US Forest Service in Alaska doing a Forest Inventory and Assessment. She also worked in Northern California where she now has her research area studying water stress at the top of Redwood trees.
Lissy illustrated how the stems of the tree branches can get embolisms (air bubbles) from high water stress. She climbs the trees using a jumar system and then brings the branches home to Flagstaff to study the hydraulic conductivity of the tree.
Lissy asked the students why the pine needles and branches might be ore water stressed at the top of the trees than down below. Even though the base of the redwood is in an often moist environment, the tops of the trees are more exposed to sun; but the primary reason is that water is pulled all the way up the xylem (water tubes) of the tree from the ground to 250-400 feet! So the top of the tree is more like a dry climate.
Lissy took the students into the laboratory where she measures the hydraulic conductivity of the branches. She had them assist her with several experimental procedures she uses to determine levels of water stress in the redwood trees.
She told us: We measure hydraulic conductivity on the hydraulic line, a system of tubes that connects a stem segment to an upstream reservoir of solution (water with a tiny bit of potassium chloride)suspended a meter above the sample, and a downstream balance. The water flowing through the sample is measured as it gets to the balance. We use this measurement to infer about the degree of embolism, or air bubbles blocking the flow of water, present in the xylem cells. If the conductivity is very low, for example, we presume that there are a lot of embolisms, and the tree the sample came from was subject to a lot of water stress.
In the image above, a student measures pressure for another experiment. Lissy explains: When a piece of a plant is clipped off, the water inside sucks back away from the cut surface. This is because there is tension in the water in plants. If the plant is more water stressed, the tension is greater. We can measure this in units of pressure with a pressure chamber. We first cut a sample, then insert it into the chamber with the cut end extending out of the hole in the top. When we turn up the pressure in the chamber, the water will be forced back to the cut surface. We measure the pressure at which the water reaches the surface, and know that that number is equal to the tension that existed in the water in the plant before we cut it. If it takes a lot of pressure, then the plant was very water stressed.
Thank you, Lissy, for the wonderful presentation and the engaging hands-on research in your lab!
Guest Blog Post by Karin Wadsack and Todd Traen, with an update from Jenna Samora
On Friday, April 28th, nearly 100 middle and high school students competed in the first Arizona KidWind Challenge wind turbine design competition. 20 teams of students came from Sinagua Middle School, Mount Elden Middle School, STAR School, Winslow High, Coconino High, and Northland Preparatory Academy. The teams brought a wind turbine they had designed and built ahead of time to test in a wind tunnel, determining whose turbine made the most electricity over a 30-second test period. The teams also competed based on their turbine design, technical presentation, technical design knowledge, and general wind energy knowledge. The teams each met with a group of judges from the wind industry, giving a presentation about their project and answering specific design and knowledge questions.
The teams also competed in “instant challenges,” building sail cars, windmills for weight lifting, and playing wind energy Jeopardy. Throughout the day, students got to interact with other students from different schools and grade levels, and explain their own projects to peers, teachers, coaches, and visiting guests.Turbines at the competition included vertical and horizontal axis turbines, systems with and without gears, and some turbines for which the students had wound their own generators.
Frequently heard: “This is AWESOME!” “Check out that design!” “I’m having SO MUCH fun!” “Next year we’re going to do _____!”
The Wind for Schools project staff of eight was supported by an additional eight amazing volunteers from the Institute for Tribal Environmental Professionals and the Climate Science and Solutions professional master’s program at NAU. The Expert Judges also volunteered their day to the event. The Boys & Girls Club of Flagstaff generously donated its facilities for the day.
Update from Jenna Samora on the MITe Team's trip to Nationals: The Mustang Gust Runners ended up taking 1st in the Vertical Axis Insta-Challenge, but did not score high on the original wind turbine design. After the first competition in Flagstaff, the students 3D-printed their own gears and created their own generator. However, they were unable to get the energy output that they hoped for, so they went back to using the KidWind generator. Even through their turbine was not the best design, the boys still learned a lot and had a great time!
Thank you to our dedicated judges!
Ross Taylor, Wind Subject Matter Expert
Ken Kotalik, Primus Wind Power
Jim Corning, Prometheus Renewables
Daniel Snyder, Westwind Solar Inc
Darrin Russell, Wind Subject Matter Expert
Flagstaff STEM Coordinator